- Both DNA and primer should be pure and free of EDTA, Protein, RNA, Ethanol and Salt. Please DON'T suspend Primer in TE buffer. We prefer elution in sterile de-ionized water, instead of EB buffer, if you are using a Qiagen MiniPrep kit for purification.
- Primers should be in the range of 18-25 bp with 40-60% GC content.
- Please fill in a sample information file along with your samples. Please include a separate sheet for each plate. Leave plate row and col blank if samples are in tubes. Number tubes according to the first column. See here for more information.