Next Generation Sequencing
|Library Type||Max number of samples that can be multiplexed in one lane||WVU Users||Non-WVU Users|
|Each Sample||Each Sample|
|TruSeq PCR-Free DNA||24||$70||$115|
|TruSeq Small RNA||48||$100||$150|
Not sure which library prep is best for you? Click here.
|Run Type||Length||WVU User||Non-WVU User|
We have a collaborative relationship with the Marshall Genomics Core Facility which provides Illumina HiSeq sequencing for libraries prepared in our facility. Please inquire for pricing.
Other Sequencing Services
|Service Level||Single Sample*||Group of 16 Samples**||Group of 96 Samples**|
|Full Service Sequencing - includes sequencing reaction†, cleanup, and run on 3130xl||$6.50||$80||$350|
|Cleanup and Sequencing on 3130xl reaction performed by user||$4||$32||$124|
|Sequencing on 3130xl cleanup and reaction performed by user||$3||$18||$90|
|Run Fragment Analysis Samples on 3130xl User performs reaction and provides size standard||$3||$18||$90|
|RNA Nano 6000 Analysis on Agilent 2100 Bioanalyzer‡||$60/12 sample chip
|Bioanalyzer High Sensetivity DNA Chip‡||$75/11 sample chip
|Covaris DNA shearing||$6 WVU
|Illumina Library QC via qPCR||$100/1‑12 samples WVU
$150/1‑12 samples non‑WVU
* All samples that are not submitted in multiples of 16 will be charged the single sample price (e.g., if 19 samples are submitted, 16 will be
charged at the bulk rate and 3 will be charged at the single sample rate). Such samples may experience a processing delay. If expedited
processing is required, an additional fee of $15 can be added.
** We ask that you leave one well open for us to run a positive control.
† Assumes M13 or other standard primers. Custom primers should be provided by individual researchers. Submission conditions vary
depending on sample type. Please contact the lab manager for details.
‡ Please provide at least one week of advance notice for use of BioAnalyzer Chips to allow time for ordering, if necessary.
|M13_Forward (-20)  ||GTAAAACGACGGCCAGT|
|LKO.15-Human U6 promoter||GACTATCATATGCTTACCGT|
All Bioinformatic services are charged at an hourly rate of $30/hr for WVU users and $45/hr for external users. This time includes just the time a Bioinformatician is actively engaged with your data. Computer run time is not charged at this time. Below is a list of common tasks, and an estimate for how long they will take. Note that this is by no means a comprehensive list of what we can do, its just a few easily quantifyable tasks. A few things we can also help with include:
- Experiment Design
- Grant Writing
- Large Data Sets
- Mining Public Databases
- Automate Repetative Tasks
- Publication Quality Figures
- Interactive Visualizations
- Custom Analyses
|  With reference genome in Ensembl, one comparison, identified samples, less than 16 samples||6|
|Extra Comparisons||+ 1|
|Without a reference||20-40|
|16S Metagenomics: classify strains, PCoA, rarefaction, diversity||5|
|Fungal ITS: classify strains, PCoA, rarefaction, diversity||5|
|Identify significantly different species||+ 2|
Note: Bioinformatician can easily be bribed with chocolate
All prices subject to change without noticeThis is a non-profit center. We charge to cover our costs, nothing more.
Last updated February, 2013